Construct: shRNA TRCN0000072135
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010804.2-2020s1c1
- DNA Barcode:
- CCCATATGTATGGCAGTAGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mllt10 (17354)
- Gene Description:
- myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
- Transcript:
- RefSeq NM_010804.2 (NON-CURRENT)
- Match location:
- Position 2020 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000072135)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCATATGTATGGCAGTAGAT
- Hairpin Sequence:
-
5'-CCGG-CCCATATGTATGGCAGTAGAT-CTCGAG-ATCTACTGCCATACATATGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCATATGTATGGCAGTAGATCTCGAGATCTACTGCCATACATATGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCATATGTATGGCAGTAGATCTCGAGATCTACTGCCATACATATGGG-3'
Other clones with same target sequence:
(none)
