Construct: shRNA TRCN0000072244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_224s1c1
DNA Barcode:
ATCACAGAATCGTCGTATGCA

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (LUCIFERASE)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCACAGAATCGTCGTATGCA
Hairpin Sequence:
5'-CCGG-ATCACAGAATCGTCGTATGCA-CTCGAG-TGCATACGACGATTCTGTGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCACAGAATCGTCGTATGCACTCGAGTGCATACGACGATTCTGTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCACAGAATCGTCGTATGCACTCGAGTGCATACGACGATTCTGTGAT-3'

Other clones with same target sequence:

  1. TRCN0000231695