Construct: shRNA TRCN0000072294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007043.5-259s1c1
DNA Barcode:
GCCTTAAATGAACATCATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRR1 (11103)
Gene Description:
KRR1 small subunit processome component homolog
Transcript:
RefSeq NM_007043.5 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTAAATGAACATCATGTT
Hairpin Sequence:
5'-CCGG-GCCTTAAATGAACATCATGTT-CTCGAG-AACATGATGTTCATTTAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTAAATGAACATCATGTTCTCGAGAACATGATGTTCATTTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTAAATGAACATCATGTTCTCGAGAACATGATGTTCATTTAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000306840