Construct: shRNA TRCN0000072297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007043.5-968s1c1
DNA Barcode:
ACAAAGCATTTATTCCACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRR1 (11103)
Gene Description:
KRR1 small subunit processome component homolog
Transcript:
RefSeq NM_007043.5 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAAGCATTTATTCCACCTA
Hairpin Sequence:
5'-CCGG-ACAAAGCATTTATTCCACCTA-CTCGAG-TAGGTGGAATAAATGCTTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAAGCATTTATTCCACCTACTCGAGTAGGTGGAATAAATGCTTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAAGCATTTATTCCACCTACTCGAGTAGGTGGAATAAATGCTTTGT-3'

Other clones with same target sequence:

  1. TRCN0000289497