Construct: shRNA TRCN0000072323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018048.2-929s1c1
DNA Barcode:
CCTTCATACATGATTGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGOHB (55110)
Gene Description:
mago homolog B, exon junction complex subunit
Transcript:
RefSeq NM_018048.2 (NON-CURRENT)
Match location:
Position 929 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCATACATGATTGGATTT
Hairpin Sequence:
5'-CCGG-CCTTCATACATGATTGGATTT-CTCGAG-AAATCCAATCATGTATGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCATACATGATTGGATTTCTCGAGAAATCCAATCATGTATGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCATACATGATTGGATTTCTCGAGAAATCCAATCATGTATGAAGG-3'

Other clones with same target sequence:

(none)