Construct: shRNA TRCN0000072378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138285.3-1136s1c1
DNA Barcode:
GCTGGTTCCTTCGGTTAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUP35 (129401)
Gene Description:
nucleoporin 35
Transcript:
RefSeq NM_138285.3 (NON-CURRENT)
Match location:
Position 1136 (3UTR)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTCCTTCGGTTAGTTA
Hairpin Sequence:
5'-CCGG-GCTGGTTCCTTCGGTTAGTTA-CTCGAG-TAACTAACCGAAGGAACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTCCTTCGGTTAGTTACTCGAGTAACTAACCGAAGGAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTCCTTCGGTTAGTTACTCGAGTAACTAACCGAAGGAACCAGC-3'

Other clones with same target sequence:

(none)