Construct: shRNA TRCN0000072385

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001424.3-329s1c1
DNA Barcode:
CACGAATTGCACAGTCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EMP2 (2013)
Gene Description:
epithelial membrane protein 2
Transcript:
RefSeq NM_001424.3 (NON-CURRENT)
Match location:
Position 329 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072385)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGAATTGCACAGTCATCAA
Hairpin Sequence:
5'-CCGG-CACGAATTGCACAGTCATCAA-CTCGAG-TTGATGACTGTGCAATTCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGAATTGCACAGTCATCAACTCGAGTTGATGACTGTGCAATTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGAATTGCACAGTCATCAACTCGAGTTGATGACTGTGCAATTCGTG-3'

Other clones with same target sequence:

(none)