Construct: shRNA TRCN0000072400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006000.1-368s1c1
DNA Barcode:
GCCCGTGGTCACTATACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBA4A (7277)
Gene Description:
tubulin alpha 4a
Transcript:
RefSeq NM_006000.1 (NON-CURRENT)
Match location:
Position 368 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTGGTCACTATACCATT
Hairpin Sequence:
5'-CCGG-GCCCGTGGTCACTATACCATT-CTCGAG-AATGGTATAGTGACCACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTGGTCACTATACCATTCTCGAGAATGGTATAGTGACCACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTGGTCACTATACCATTCTCGAGAATGGTATAGTGACCACGGGC-3'

Other clones with same target sequence:

(none)