Construct: shRNA TRCN0000072415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_213674.1-971s1c1
DNA Barcode:
GTCTGTGGCAAAGTTGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPM2 (7169)
Gene Description:
tropomyosin 2
Transcript:
RefSeq NM_213674.1 (CURRENT)
Match location:
Position 971 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGTGGCAAAGTTGGAGAA
Hairpin Sequence:
5'-CCGG-GTCTGTGGCAAAGTTGGAGAA-CTCGAG-TTCTCCAACTTTGCCACAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGTGGCAAAGTTGGAGAACTCGAGTTCTCCAACTTTGCCACAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGTGGCAAAGTTGGAGAACTCGAGTTCTCCAACTTTGCCACAGAC-3'

Other clones with same target sequence:

(none)