Construct: shRNA TRCN0000072443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004280.2-615s1c1
DNA Barcode:
CCACAGGACTAATGTAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF1E1 (9521)
Gene Description:
eukaryotic translation elongation factor 1 epsilon 1
Transcript:
RefSeq NM_004280.2 (NON-CURRENT)
Match location:
Position 615 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGGACTAATGTAAATTA
Hairpin Sequence:
5'-CCGG-CCACAGGACTAATGTAAATTA-CTCGAG-TAATTTACATTAGTCCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGGACTAATGTAAATTACTCGAGTAATTTACATTAGTCCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGGACTAATGTAAATTACTCGAGTAATTTACATTAGTCCTGTGG-3'

Other clones with same target sequence:

(none)