Construct: shRNA TRCN0000072525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033137.1-219s1c1
DNA Barcode:
CAAACTCCTCTACTGTAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGF1 (2246)
Gene Description:
fibroblast growth factor 1
Transcript:
RefSeq NM_033137.1 (NON-CURRENT)
Match location:
Position 219 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACTCCTCTACTGTAGCAA
Hairpin Sequence:
5'-CCGG-CAAACTCCTCTACTGTAGCAA-CTCGAG-TTGCTACAGTAGAGGAGTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACTCCTCTACTGTAGCAACTCGAGTTGCTACAGTAGAGGAGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACTCCTCTACTGTAGCAACTCGAGTTGCTACAGTAGAGGAGTTTG-3'

Other clones with same target sequence:

(none)