Construct: shRNA TRCN0000072544

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016391.2-901s1c1
DNA Barcode:
CGGAGTAAGATCAACGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOP16 (51491)
Gene Description:
NOP16 nucleolar protein
Transcript:
RefSeq NM_016391.2 (NON-CURRENT)
Match location:
Position 901 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072544)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGTAAGATCAACGTCTAT
Hairpin Sequence:
5'-CCGG-CGGAGTAAGATCAACGTCTAT-CTCGAG-ATAGACGTTGATCTTACTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGTAAGATCAACGTCTATCTCGAGATAGACGTTGATCTTACTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGTAAGATCAACGTCTATCTCGAGATAGACGTTGATCTTACTCCG-3'

Other clones with same target sequence:

  1. TRCN0000286128