Construct: shRNA TRCN0000072547

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016391.2-489s1c1
DNA Barcode:
GAAGTTTGGTTACAGTGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOP16 (51491)
Gene Description:
NOP16 nucleolar protein
Transcript:
RefSeq NM_016391.2 (NON-CURRENT)
Match location:
Position 489 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072547)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTTTGGTTACAGTGTCAA
Hairpin Sequence:
5'-CCGG-GAAGTTTGGTTACAGTGTCAA-CTCGAG-TTGACACTGTAACCAAACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTTTGGTTACAGTGTCAACTCGAGTTGACACTGTAACCAAACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTTTGGTTACAGTGTCAACTCGAGTTGACACTGTAACCAAACTTC-3'

Other clones with same target sequence:

  1. TRCN0000307890