Construct: shRNA TRCN0000072602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005954.2-284s1c1
DNA Barcode:
CGCGGACTCCTGCAAGTGCGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT3 (4504)
Gene Description:
metallothionein 3
Transcript:
RefSeq NM_005954.2 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGGACTCCTGCAAGTGCGA
Hairpin Sequence:
5'-CCGG-CGCGGACTCCTGCAAGTGCGA-CTCGAG-TCGCACTTGCAGGAGTCCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGGACTCCTGCAAGTGCGACTCGAGTCGCACTTGCAGGAGTCCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGGACTCCTGCAAGTGCGACTCGAGTCGCACTTGCAGGAGTCCGCG-3'

Other clones with same target sequence:

(none)