Construct: shRNA TRCN0000072616

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014018.2-271s1c1
DNA Barcode:
TGCTGAGACATTCTCCTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPS28 (28957)
Gene Description:
mitochondrial ribosomal protein S28
Transcript:
RefSeq NM_014018.2 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072616)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGAGACATTCTCCTCTTA
Hairpin Sequence:
5'-CCGG-TGCTGAGACATTCTCCTCTTA-CTCGAG-TAAGAGGAGAATGTCTCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGAGACATTCTCCTCTTACTCGAGTAAGAGGAGAATGTCTCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGAGACATTCTCCTCTTACTCGAGTAAGAGGAGAATGTCTCAGCA-3'

Other clones with same target sequence:

  1. TRCN0000291930