Construct: shRNA TRCN0000072683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_095991.7-2448s1c1
DNA Barcode:
GCAAAGAAACTAGGGAAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP78 (84131)
Gene Description:
centrosomal protein 78
Transcript:
RefSeq XM_095991.7 (NON-CURRENT)
Match location:
Position 2448 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGAAACTAGGGAAACTA
Hairpin Sequence:
5'-CCGG-GCAAAGAAACTAGGGAAACTA-CTCGAG-TAGTTTCCCTAGTTTCTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGAAACTAGGGAAACTACTCGAGTAGTTTCCCTAGTTTCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGAAACTAGGGAAACTACTCGAGTAGTTTCCCTAGTTTCTTTGC-3'

Other clones with same target sequence:

(none)