Construct: shRNA TRCN0000072720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032359.2-462s1c1
DNA Barcode:
CGCTTGGTGATTGAATTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMSS1 (84319)
Gene Description:
cms1 ribosomal small subunit homolog
Transcript:
RefSeq NM_032359.2 (NON-CURRENT)
Match location:
Position 462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTGGTGATTGAATTAGAA
Hairpin Sequence:
5'-CCGG-CGCTTGGTGATTGAATTAGAA-CTCGAG-TTCTAATTCAATCACCAAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTGGTGATTGAATTAGAACTCGAGTTCTAATTCAATCACCAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTGGTGATTGAATTAGAACTCGAGTTCTAATTCAATCACCAAGCG-3'

Other clones with same target sequence:

(none)