Construct: shRNA TRCN0000072730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022338.2-1186s1c1
DNA Barcode:
GCCTGTGGTTAACACAACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C11orf24 (53838)
Gene Description:
chromosome 11 open reading frame 24
Transcript:
RefSeq NM_022338.2 (NON-CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTGGTTAACACAACAAA
Hairpin Sequence:
5'-CCGG-GCCTGTGGTTAACACAACAAA-CTCGAG-TTTGTTGTGTTAACCACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTGGTTAACACAACAAACTCGAGTTTGTTGTGTTAACCACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTGGTTAACACAACAAACTCGAGTTTGTTGTGTTAACCACAGGC-3'

Other clones with same target sequence:

  1. TRCN0000307742