Construct: shRNA TRCN0000072820

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_117174.6-799s1c1
DNA Barcode:
CCCGATGCTTTCCACATTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC5A9 (200010)
Gene Description:
solute carrier family 5 member 9
Transcript:
RefSeq XM_117174.6 (NON-CURRENT)
Match location:
Position 799 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072820)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGATGCTTTCCACATTCTT
Hairpin Sequence:
5'-CCGG-CCCGATGCTTTCCACATTCTT-CTCGAG-AAGAATGTGGAAAGCATCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGATGCTTTCCACATTCTTCTCGAGAAGAATGTGGAAAGCATCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGATGCTTTCCACATTCTTCTCGAGAAGAATGTGGAAAGCATCGGG-3'

Other clones with same target sequence:

(none)