Construct: shRNA TRCN0000072868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005490.1-2083s1c1
DNA Barcode:
CAAATGTGACTCCACCTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH2D3A (10045)
Gene Description:
SH2 domain containing 3A
Transcript:
RefSeq NM_005490.1 (NON-CURRENT)
Match location:
Position 2083 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATGTGACTCCACCTCTTA
Hairpin Sequence:
5'-CCGG-CAAATGTGACTCCACCTCTTA-CTCGAG-TAAGAGGTGGAGTCACATTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATGTGACTCCACCTCTTACTCGAGTAAGAGGTGGAGTCACATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATGTGACTCCACCTCTTACTCGAGTAAGAGGTGGAGTCACATTTG-3'

Other clones with same target sequence:

(none)