Construct: shRNA TRCN0000072871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005490.1-594s1c1
DNA Barcode:
AGCCTGCAGATTTGGCACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH2D3A (10045)
Gene Description:
SH2 domain containing 3A
Transcript:
RefSeq NM_005490.1 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTGCAGATTTGGCACATA
Hairpin Sequence:
5'-CCGG-AGCCTGCAGATTTGGCACATA-CTCGAG-TATGTGCCAAATCTGCAGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTGCAGATTTGGCACATACTCGAGTATGTGCCAAATCTGCAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTGCAGATTTGGCACATACTCGAGTATGTGCCAAATCTGCAGGCT-3'

Other clones with same target sequence:

(none)