Construct: shRNA TRCN0000073115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032373.2-978s1c1
DNA Barcode:
GTATCGACCAAGAATTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCGF5 (84333)
Gene Description:
polycomb group ring finger 5
Transcript:
RefSeq NM_032373.2 (NON-CURRENT)
Match location:
Position 978 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCGACCAAGAATTGATTT
Hairpin Sequence:
5'-CCGG-GTATCGACCAAGAATTGATTT-CTCGAG-AAATCAATTCTTGGTCGATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCGACCAAGAATTGATTTCTCGAGAAATCAATTCTTGGTCGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCGACCAAGAATTGATTTCTCGAGAAATCAATTCTTGGTCGATAC-3'

Other clones with same target sequence:

(none)