Construct: shRNA TRCN0000073125

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032622.1-588s1c1
DNA Barcode:
CCACTATTAGAAGCAGATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LNX1 (84708)
Gene Description:
ligand of numb-protein X 1
Transcript:
RefSeq NM_032622.1 (NON-CURRENT)
Match location:
Position 588 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073125)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTATTAGAAGCAGATCAT
Hairpin Sequence:
5'-CCGG-CCACTATTAGAAGCAGATCAT-CTCGAG-ATGATCTGCTTCTAATAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTATTAGAAGCAGATCATCTCGAGATGATCTGCTTCTAATAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTATTAGAAGCAGATCATCTCGAGATGATCTGCTTCTAATAGTGG-3'

Other clones with same target sequence:

(none)