Construct: shRNA TRCN0000073149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032873.2-930s1c1
DNA Barcode:
CCTCATAAGAAGCAGCTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBASH3B (84959)
Gene Description:
ubiquitin associated and SH3 domain containing B
Transcript:
RefSeq NM_032873.2 (NON-CURRENT)
Match location:
Position 930 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATAAGAAGCAGCTACAT
Hairpin Sequence:
5'-CCGG-CCTCATAAGAAGCAGCTACAT-CTCGAG-ATGTAGCTGCTTCTTATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATAAGAAGCAGCTACATCTCGAGATGTAGCTGCTTCTTATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATAAGAAGCAGCTACATCTCGAGATGTAGCTGCTTCTTATGAGG-3'

Other clones with same target sequence:

(none)