Construct: shRNA TRCN0000073154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138361.2-620s1c1
DNA Barcode:
GCAACCATCAAGGTTCTAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRSAM1 (90678)
Gene Description:
leucine rich repeat and sterile alpha motif containing 1
Transcript:
RefSeq NM_138361.2 (NON-CURRENT)
Match location:
Position 620 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCATCAAGGTTCTAGAT
Hairpin Sequence:
5'-CCGG-GCAACCATCAAGGTTCTAGAT-CTCGAG-ATCTAGAACCTTGATGGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCATCAAGGTTCTAGATCTCGAGATCTAGAACCTTGATGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCATCAAGGTTCTAGATCTCGAGATCTAGAACCTTGATGGTTGC-3'

Other clones with same target sequence:

(none)