Construct: shRNA TRCN0000073177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178450.2-920s1c1
DNA Barcode:
CAGAGGGTGATTCTCCTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCHF3 (115123)
Gene Description:
membrane associated ring-CH-type finger 3
Transcript:
RefSeq NM_178450.2 (NON-CURRENT)
Match location:
Position 920 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGGGTGATTCTCCTCATT
Hairpin Sequence:
5'-CCGG-CAGAGGGTGATTCTCCTCATT-CTCGAG-AATGAGGAGAATCACCCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGGGTGATTCTCCTCATTCTCGAGAATGAGGAGAATCACCCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGGGTGATTCTCCTCATTCTCGAGAATGAGGAGAATCACCCTCTG-3'

Other clones with same target sequence:

(none)