Construct: shRNA TRCN0000073213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152598.1-2806s1c1
DNA Barcode:
CAAAGCGTTCACAGTTAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCHF10 (162333)
Gene Description:
membrane associated ring-CH-type finger 10
Transcript:
RefSeq NM_152598.1 (NON-CURRENT)
Match location:
Position 2806 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGCGTTCACAGTTAGATT
Hairpin Sequence:
5'-CCGG-CAAAGCGTTCACAGTTAGATT-CTCGAG-AATCTAACTGTGAACGCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGCGTTCACAGTTAGATTCTCGAGAATCTAACTGTGAACGCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGCGTTCACAGTTAGATTCTCGAGAATCTAACTGTGAACGCTTTG-3'

Other clones with same target sequence:

(none)