Construct: shRNA TRCN0000073242

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198849.1-372s1c1
DNA Barcode:
CTGCGGCAGATCCATAGGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIAH3 (283514)
Gene Description:
siah E3 ubiquitin protein ligase family member 3
Transcript:
RefSeq NM_198849.1 (NON-CURRENT)
Match location:
Position 372 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073242)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGGCAGATCCATAGGGTT
Hairpin Sequence:
5'-CCGG-CTGCGGCAGATCCATAGGGTT-CTCGAG-AACCCTATGGATCTGCCGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGGCAGATCCATAGGGTTCTCGAGAACCCTATGGATCTGCCGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGGCAGATCCATAGGGTTCTCGAGAACCCTATGGATCTGCCGCAG-3'

Other clones with same target sequence:

(none)