Construct: shRNA TRCN0000073248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152616.3-2386s1c1
DNA Barcode:
CCTCAGACTCATCACAAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM42 (287015)
Gene Description:
tripartite motif containing 42
Transcript:
RefSeq NM_152616.3 (NON-CURRENT)
Match location:
Position 2386 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGACTCATCACAAAGTA
Hairpin Sequence:
5'-CCGG-CCTCAGACTCATCACAAAGTA-CTCGAG-TACTTTGTGATGAGTCTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGACTCATCACAAAGTACTCGAGTACTTTGTGATGAGTCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGACTCATCACAAAGTACTCGAGTACTTTGTGATGAGTCTGAGG-3'

Other clones with same target sequence:

(none)