Construct: shRNA TRCN0000073279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002105.2-289s1c1
DNA Barcode:
CGACAACAAGAAGACGCGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AX (3014)
Gene Description:
H2A.X variant histone
Transcript:
RefSeq NM_002105.2 (CURRENT)
Match location:
Position 289 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAACAAGAAGACGCGAAT
Hairpin Sequence:
5'-CCGG-CGACAACAAGAAGACGCGAAT-CTCGAG-ATTCGCGTCTTCTTGTTGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAACAAGAAGACGCGAATCTCGAGATTCGCGTCTTCTTGTTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAACAAGAAGACGCGAATCTCGAGATTCGCGTCTTCTTGTTGTCG-3'

Other clones with same target sequence:

(none)