Construct: shRNA TRCN0000073318
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032875.1-2128s1c1
- DNA Barcode:
- CCTAAATGGTTGCCTTGAAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FBXL20 (84961)
- Gene Description:
- F-box and leucine rich repeat protein 20
- Transcript:
- RefSeq NM_032875.1 (NON-CURRENT)
- Match location:
- Position 2128 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000073318)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTAAATGGTTGCCTTGAAAT
- Hairpin Sequence:
-
5'-CCGG-CCTAAATGGTTGCCTTGAAAT-CTCGAG-ATTTCAAGGCAACCATTTAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTAAATGGTTGCCTTGAAATCTCGAGATTTCAAGGCAACCATTTAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTAAATGGTTGCCTTGAAATCTCGAGATTTCAAGGCAACCATTTAGG-3'
Other clones with same target sequence:
-
TRCN0000327649
