Construct: shRNA TRCN0000073321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032875.1-1312s1c1
DNA Barcode:
GCCCACTAATCACAGATGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL20 (84961)
Gene Description:
F-box and leucine rich repeat protein 20
Transcript:
RefSeq NM_032875.1 (NON-CURRENT)
Match location:
Position 1312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACTAATCACAGATGCAT
Hairpin Sequence:
5'-CCGG-GCCCACTAATCACAGATGCAT-CTCGAG-ATGCATCTGTGATTAGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACTAATCACAGATGCATCTCGAGATGCATCTGTGATTAGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACTAATCACAGATGCATCTCGAGATGCATCTGTGATTAGTGGGC-3'

Other clones with same target sequence:

(none)