Construct: shRNA TRCN0000073368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033254.2-4140s1c1
DNA Barcode:
CCCATGAGAACAGACCAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BOC (91653)
Gene Description:
BOC cell adhesion associated, oncogene regulated
Transcript:
RefSeq NM_033254.2 (NON-CURRENT)
Match location:
Position 4140 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGAGAACAGACCAAGAT
Hairpin Sequence:
5'-CCGG-CCCATGAGAACAGACCAAGAT-CTCGAG-ATCTTGGTCTGTTCTCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGAGAACAGACCAAGATCTCGAGATCTTGGTCTGTTCTCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGAGAACAGACCAAGATCTCGAGATCTTGGTCTGTTCTCATGGG-3'

Other clones with same target sequence:

(none)