Construct: shRNA TRCN0000073387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020693.2-3062s1c1
DNA Barcode:
CCGCATGTACTCTTTCAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DSCAML1 (57453)
Gene Description:
DS cell adhesion molecule like 1
Transcript:
RefSeq NM_020693.2 (NON-CURRENT)
Match location:
Position 3062 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCATGTACTCTTTCAACAA
Hairpin Sequence:
5'-CCGG-CCGCATGTACTCTTTCAACAA-CTCGAG-TTGTTGAAAGAGTACATGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCATGTACTCTTTCAACAACTCGAGTTGTTGAAAGAGTACATGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCATGTACTCTTTCAACAACTCGAGTTGTTGAAAGAGTACATGCGG-3'

Other clones with same target sequence:

(none)