Construct: shRNA TRCN0000073433

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015541.1-2551s1c1
DNA Barcode:
GCCGGTTCTATTTCAGCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRIG1 (26018)
Gene Description:
leucine rich repeats and immunoglobulin like domains 1
Transcript:
RefSeq NM_015541.1 (NON-CURRENT)
Match location:
Position 2551 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073433)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGTTCTATTTCAGCTAAT
Hairpin Sequence:
5'-CCGG-GCCGGTTCTATTTCAGCTAAT-CTCGAG-ATTAGCTGAAATAGAACCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGTTCTATTTCAGCTAATCTCGAGATTAGCTGAAATAGAACCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGTTCTATTTCAGCTAATCTCGAGATTAGCTGAAATAGAACCGGC-3'

Other clones with same target sequence:

  1. TRCN0000289844