Construct: shRNA TRCN0000073445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006614.2-1586s1c1
DNA Barcode:
CCATTGATACAAACCAAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHL1 (10752)
Gene Description:
cell adhesion molecule L1 like
Transcript:
RefSeq NM_006614.2 (NON-CURRENT)
Match location:
Position 1586 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGATACAAACCAAAGAT
Hairpin Sequence:
5'-CCGG-CCATTGATACAAACCAAAGAT-CTCGAG-ATCTTTGGTTTGTATCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGATACAAACCAAAGATCTCGAGATCTTTGGTTTGTATCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGATACAAACCAAAGATCTCGAGATCTTTGGTTTGTATCAATGG-3'

Other clones with same target sequence:

(none)