Construct: shRNA TRCN0000073458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000615.1-2796s1c1
DNA Barcode:
GCTTCACCAGAGCATTTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCAM1 (4684)
Gene Description:
neural cell adhesion molecule 1
Transcript:
RefSeq NM_000615.1 (NON-CURRENT)
Match location:
Position 2796 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCACCAGAGCATTTCCAA
Hairpin Sequence:
5'-CCGG-GCTTCACCAGAGCATTTCCAA-CTCGAG-TTGGAAATGCTCTGGTGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCACCAGAGCATTTCCAACTCGAGTTGGAAATGCTCTGGTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCACCAGAGCATTTCCAACTCGAGTTGGAAATGCTCTGGTGAAGC-3'

Other clones with same target sequence:

(none)