Construct: shRNA TRCN0000073463

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016081.2-4451s1c1
DNA Barcode:
GCTCTGAATAAAGCAGAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PALLD (23022)
Gene Description:
palladin, cytoskeletal associated protein
Transcript:
RefSeq NM_016081.2 (NON-CURRENT)
Match location:
Position 4451 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073463)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGAATAAAGCAGAAATA
Hairpin Sequence:
5'-CCGG-GCTCTGAATAAAGCAGAAATA-CTCGAG-TATTTCTGCTTTATTCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGAATAAAGCAGAAATACTCGAGTATTTCTGCTTTATTCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGAATAAAGCAGAAATACTCGAGTATTTCTGCTTTATTCAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000289995