Construct: shRNA TRCN0000073488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006093.2-716s1c1
DNA Barcode:
CCTATGCACCAAAGGAGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRG3 (10394)
Gene Description:
proteoglycan 3, pro eosinophil major basic protein 2
Transcript:
RefSeq NM_006093.2 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATGCACCAAAGGAGGTTA
Hairpin Sequence:
5'-CCGG-CCTATGCACCAAAGGAGGTTA-CTCGAG-TAACCTCCTTTGGTGCATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATGCACCAAAGGAGGTTACTCGAGTAACCTCCTTTGGTGCATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATGCACCAAAGGAGGTTACTCGAGTAACCTCCTTTGGTGCATAGG-3'

Other clones with same target sequence:

(none)