Construct: shRNA TRCN0000073499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006846.1-1466s1c1
DNA Barcode:
GCCTTCTTTCAACAAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPINK5 (11005)
Gene Description:
serine peptidase inhibitor Kazal type 5
Transcript:
RefSeq NM_006846.1 (NON-CURRENT)
Match location:
Position 1466 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTTTCAACAAGAAGAA
Hairpin Sequence:
5'-CCGG-GCCTTCTTTCAACAAGAAGAA-CTCGAG-TTCTTCTTGTTGAAAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTTTCAACAAGAAGAACTCGAGTTCTTCTTGTTGAAAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTTTCAACAAGAAGAACTCGAGTTCTTCTTGTTGAAAGAAGGC-3'

Other clones with same target sequence:

(none)