Construct: shRNA TRCN0000073521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016192.2-978s1c1
DNA Barcode:
GCGCTTCTGATGGGAAATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEFF2 (23671)
Gene Description:
transmembrane protein with EGF like and two follistatin like domains 2
Transcript:
RefSeq NM_016192.2 (NON-CURRENT)
Match location:
Position 978 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTTCTGATGGGAAATCTT
Hairpin Sequence:
5'-CCGG-GCGCTTCTGATGGGAAATCTT-CTCGAG-AAGATTTCCCATCAGAAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTTCTGATGGGAAATCTTCTCGAGAAGATTTCCCATCAGAAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTTCTGATGGGAAATCTTCTCGAGAAGATTTCCCATCAGAAGCGC-3'

Other clones with same target sequence:

(none)