Construct: shRNA TRCN0000073638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001750.4-2257s1c1
DNA Barcode:
GTATCTGGTATCTGCATGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAST (831)
Gene Description:
calpastatin
Transcript:
RefSeq NM_001750.4 (NON-CURRENT)
Match location:
Position 2257 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCTGGTATCTGCATGTAA
Hairpin Sequence:
5'-CCGG-GTATCTGGTATCTGCATGTAA-CTCGAG-TTACATGCAGATACCAGATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCTGGTATCTGCATGTAACTCGAGTTACATGCAGATACCAGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCTGGTATCTGCATGTAACTCGAGTTACATGCAGATACCAGATAC-3'

Other clones with same target sequence:

  1. TRCN0000333790