Construct: shRNA TRCN0000073659

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015692.1-2259s1c1
DNA Barcode:
CCCGAAACATGGATTTGGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPAMD8 (27151)
Gene Description:
C3 and PZP like alpha-2-macroglobulin domain containing 8
Transcript:
RefSeq NM_015692.1 (NON-CURRENT)
Match location:
Position 2259 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073659)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAAACATGGATTTGGCAT
Hairpin Sequence:
5'-CCGG-CCCGAAACATGGATTTGGCAT-CTCGAG-ATGCCAAATCCATGTTTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAAACATGGATTTGGCATCTCGAGATGCCAAATCCATGTTTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAAACATGGATTTGGCATCTCGAGATGCCAAATCCATGTTTCGGG-3'

Other clones with same target sequence:

(none)