Construct: shRNA TRCN0000073785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144575.2-1152s1c1
DNA Barcode:
GCCATGTTTATATGTAGCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAPN13 (92291)
Gene Description:
calpain 13
Transcript:
RefSeq NM_144575.2 (NON-CURRENT)
Match location:
Position 1152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTTATATGTAGCGAA
Hairpin Sequence:
5'-CCGG-GCCATGTTTATATGTAGCGAA-CTCGAG-TTCGCTACATATAAACATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTTATATGTAGCGAACTCGAGTTCGCTACATATAAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTTATATGTAGCGAACTCGAGTTCGCTACATATAAACATGGC-3'

Other clones with same target sequence:

(none)