Construct: shRNA TRCN0000073798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013325.3-116s1c1
DNA Barcode:
CCGTTTGGATACTGGGTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG4B (23192)
Gene Description:
autophagy related 4B cysteine peptidase
Transcript:
RefSeq NM_013325.3 (NON-CURRENT)
Match location:
Position 116 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTGGATACTGGGTAGAA
Hairpin Sequence:
5'-CCGG-CCGTTTGGATACTGGGTAGAA-CTCGAG-TTCTACCCAGTATCCAAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTGGATACTGGGTAGAACTCGAGTTCTACCCAGTATCCAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTGGATACTGGGTAGAACTCGAGTTCTACCCAGTATCCAAACGG-3'

Other clones with same target sequence:

(none)