Construct: shRNA TRCN0000073828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012291.3-6373s1c1
DNA Barcode:
GCTGTCTTATTGATGCTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ESPL1 (9700)
Gene Description:
extra spindle pole bodies like 1, separase
Transcript:
RefSeq NM_012291.3 (NON-CURRENT)
Match location:
Position 6373 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCTTATTGATGCTAGAA
Hairpin Sequence:
5'-CCGG-GCTGTCTTATTGATGCTAGAA-CTCGAG-TTCTAGCATCAATAAGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCTTATTGATGCTAGAACTCGAGTTCTAGCATCAATAAGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCTTATTGATGCTAGAACTCGAGTTCTAGCATCAATAAGACAGC-3'

Other clones with same target sequence:

  1. TRCN0000291193