Construct: shRNA TRCN0000073861
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032823.3-2143s1c1
- DNA Barcode:
- GCACAAGTTCACGAAAGCCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AOPEP (84909)
- Gene Description:
- aminopeptidase O (putative)
- Transcript:
- RefSeq NM_032823.3 (NON-CURRENT)
- Match location:
- Position 2143 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000073861)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACAAGTTCACGAAAGCCTA
- Hairpin Sequence:
-
5'-CCGG-GCACAAGTTCACGAAAGCCTA-CTCGAG-TAGGCTTTCGTGAACTTGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACAAGTTCACGAAAGCCTACTCGAGTAGGCTTTCGTGAACTTGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACAAGTTCACGAAAGCCTACTCGAGTAGGCTTTCGTGAACTTGTGC-3'
Other clones with same target sequence:
(none)
