Construct: shRNA TRCN0000073912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024783.2-1383s1c1
DNA Barcode:
GCCTAGCAGGAAATACCGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGBL2 (79841)
Gene Description:
ATP/GTP binding protein like 2
Transcript:
RefSeq NM_024783.2 (NON-CURRENT)
Match location:
Position 1383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGCAGGAAATACCGTTT
Hairpin Sequence:
5'-CCGG-GCCTAGCAGGAAATACCGTTT-CTCGAG-AAACGGTATTTCCTGCTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGCAGGAAATACCGTTTCTCGAGAAACGGTATTTCCTGCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGCAGGAAATACCGTTTCTCGAGAAACGGTATTTCCTGCTAGGC-3'

Other clones with same target sequence:

(none)