Construct: shRNA TRCN0000073925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020383.2-1137s1c1
DNA Barcode:
GCTCACATTAAAGATGCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPNPEP1 (7511)
Gene Description:
X-prolyl aminopeptidase 1
Transcript:
RefSeq NM_020383.2 (NON-CURRENT)
Match location:
Position 1137 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACATTAAAGATGCTGTT
Hairpin Sequence:
5'-CCGG-GCTCACATTAAAGATGCTGTT-CTCGAG-AACAGCATCTTTAATGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACATTAAAGATGCTGTTCTCGAGAACAGCATCTTTAATGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACATTAAAGATGCTGTTCTCGAGAACAGCATCTTTAATGTGAGC-3'

Other clones with same target sequence:

(none)