Construct: shRNA TRCN0000073945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005467.2-1960s1c1
DNA Barcode:
CGACTTATACAAGTTGATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAALAD2 (10003)
Gene Description:
N-acetylated alpha-linked acidic dipeptidase 2
Transcript:
RefSeq NM_005467.2 (NON-CURRENT)
Match location:
Position 1960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTTATACAAGTTGATCTT
Hairpin Sequence:
5'-CCGG-CGACTTATACAAGTTGATCTT-CTCGAG-AAGATCAACTTGTATAAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTTATACAAGTTGATCTTCTCGAGAAGATCAACTTGTATAAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTTATACAAGTTGATCTTCTCGAGAAGATCAACTTGTATAAGTCG-3'

Other clones with same target sequence:

(none)